Spectroscopic Characterization of the Exopolysaccharide of Xanthomonas axonopodis pv. citri in Cu2+ Resistance Mechanism
نویسندگان
چکیده
Analizou-se o papel do exopolissacarídeo (EPS) no mecanismo de resistência ao Cu da Xanthomonas axonopodis pv. citri (Xac) com as espectroscopias de infravermelho com transformada de Fourier (FTIR), ressonância paramagnética eletrônica (RPE) e ressonância magnética nuclear (RMN). Os dados de FTIR demonstraram que as células cultivadas na presença de 0.2 mmol L de CuSO4 produziram uma maior quantidade de exopolissacarídeo piruvatado do que as células cultivadas na sua ausência. Os dados de RPE indicaram que a quantidade de íons Cu diminuiu com o tempo de cultura. Os dados de RMN de C mostraram a complexação dos íons de Cu com o EPS. Esses resultados mostraram que o EPS tem um papel importante no mecanismo de proteção da Xac ao Cu e este age no mecanismo inicial de proteção, ligando-se aos íons de Cu livres, reduzindo sua difusão e o transporte ativo ao citoplasma . O Cu também induz a produção de EPS piruvatado, que aumenta a capacidade de coleta e complexação desses íons.
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